| Autor: |
Østergaard PF; Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark., Matteucci M, Reisner W, Taboryski R |
| Jazyk: |
angličtina |
| Zdroj: |
The Analyst [Analyst] 2013 Feb 21; Vol. 138 (4), pp. 1249-55. |
| DOI: |
10.1039/c2an36522g |
| Abstrakt: |
Nanochannel technology, coupled with a suitable DNA labeling chemistry, is a powerful approach for performing high-throughput single-molecule mapping of genomes. Yet so far nanochannel technology has remained inaccessible to the broader research community due to high fabrication cost and/or requirement of specialized facilities/skill-sets. In this article we show that nanochannel-based mapping can be performed in all polymer chips fabricated via injection molding: a fabrication process so inexpensive that the devices can be considered disposable. Fluorescent intensity variations can be obtained from molecules extended in the polymer nanochannels via chemical counterstaining against YOYO-1. In particular, we demonstrate that the counterstaining induced fluorescent intensity variations to a large degree appear to be proportional to the theoretically computed sequence-maps of both local AT and GC variation along DNA sequences. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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