Metabolism and protein transformations in synovial membrane of a knee joint in the course of rheumatoid arthritis and degenerative arthritis.
| Autor: | Hapeta B; Institute of General Biochemistry of the Chair of Biochemistry, Medical University of Silesia, Zabrze, Poland. beata_hapeta@wp.pl, Koczy B, Fitowska A, Dobrakowski M, Kasperczyk A, Ostałowska A, Stołtny T, Widuchowski W, Łukasik P, Birkner E, Kasperczyk S |
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| Jazyk: | angličtina |
| Zdroj: | Polish orthopedics and traumatology [Pol Orthop Traumatol] 2012 Aug 23; Vol. 77, pp. 53-8. Date of Electronic Publication: 2012 Aug 23. |
| Abstrakt: | Background: In the course of musculoskeletal system diseases, such as rheumatoid arthritis and degenerative arthritis a chronic inflammatory process develops, which deteriorates all the joint elements and leads to the movement insufficiency of a patient. In case of both of theses diseases, etiology is multi-factor and still not known thoroughly. It is suggested that in the process of degradation of a joint cartilage, active form of oxygen take part. Their excessive production contributes to oxidation imbalances in cells and an oxidative stress. Under the activity of fee radicals, among others, activation of proteolytic enzymes participating the collagen degradation starts. The aim of this work is to compare parameters characteristic a cell metabolism and protein transformations taking place in the course of the aforementioned musculoskeletal system diseases. Material/methods: The material tested consisted of fragments of synovial membrane of a knee joint taken from 36 women suffering from rheumatoid arthritis and 24 women suffering from osteoarthritis during the procedure of knee-joint endoprothesoplastic surgery. Then the material was subject to the author's methodology of preparations of synovial membrane for biochemical markings. Results: In the group of patients suffering from rheumatoid arthritis significantly higher protein and sulfhydryl groups concentrations were achieved. Moreover, an increase of activity of manganese isoenzyme of superoxide dismutase, glutamate dehydrogenase and enzymes participating in the process of collagen degradation--prolidase and acid phosphatase was observed. Conclusions: In the course of rheumatoid arthritis a speed of cell metabolism increase, which leads to a higher intensity of protein turnover in cells. |
| Databáze: | MEDLINE |
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