| Autor: |
Eckhardt TH; Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands., Skotnicka D, Kok J, Kuipers OP |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of bacteriology [J Bacteriol] 2013 Mar; Vol. 195 (5), pp. 1081-9. Date of Electronic Publication: 2012 Dec 28. |
| DOI: |
10.1128/JB.02043-12 |
| Abstrakt: |
Here we study the influence of the putative fatty acid biosynthesis (FAB) regulator FabT (originally called RmaG [Llmg_1788]) on gene transcription in Lactococcus lactis MG1363. A strain with a knockout mutation of the putative regulator was constructed, and its transcriptome was compared to that of the wild-type strain. Almost all FAB genes were significantly upregulated in the knockout. Using electrophoretic mobility shift assays (EMSAs) and DNase I footprinting, the binding motif of the regulator and the binding locations in the genome were characterized. Fatty acid composition analysis revealed that a strain lacking FabT contained significantly more saturated acyl chains in its phospholipids. This observation demonstrates that the vital pathway of FAB in L. lactis is regulated by the repressor FabT. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
