| Autor: |
Johansen LK; Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark. louise-k@life.ku.dk, Svalastoga EL, Frees D, Aalbæk B, Koch J, Iburg TM, Nielsen OL, Leifsson PS, Jensen HE |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of investigative surgery : the official journal of the Academy of Surgical Research [J Invest Surg] 2013 Jun; Vol. 26 (3), pp. 149-53. Date of Electronic Publication: 2012 Dec 28. |
| DOI: |
10.3109/08941939.2012.718043 |
| Abstrakt: |
A new inoculation technique has been developed and applied in a porcine model of juvenile hematogenous osteomyelitis. Following the success of the model, we describe the inoculation technique in detail to enable its replication in future studies. The technique was based on an anatomical feature of the femoral artery that enables inoculation into the artery using a simple surgical procedure. Inoculation in the femoral artery is advantageous because the localization of lesions constitutes a discriminative model of the naturally occurring hematogenous osteomyelitis in long bones, usually involving femur and tibia in children. The procedure was performed under general anesthesia and consisted of five major steps: (1) Exposure of the right femoral artery, (2) retrograde catheterization, (3) inoculation of bacteria, (4) hemostasis of the arterial puncture site using compression, and (5) suturing of the wound in two layers. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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