| Autor: |
Tjoeng FS; Department of Biological Sciences, Monsanto Company, Chesterfield, MO., Towery DS, Bulock JW, Whipple DE, Fok KF, Williams MH, Zupec ME, Adams SP |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of peptide and protein research [Int J Pept Protein Res] 1990 Feb; Vol. 35 (2), pp. 141-6. |
| DOI: |
10.1111/j.1399-3011.1990.tb00249.x |
| Abstrakt: |
An automated multiple peptide synthesis method to synthesize, cleave, and purify several peptides simultaneously in a single batch has been developed. The technique is based on the synthesis of multiple peptides on a single solid phase support and is easily adapted to manual or to automated methods. The approach relies on coupling of amino acid mixtures to the resin and it has been found that DCC/HOBt gives the best coupling performance. Fast Atom Bombardment Mass Spectrometry (FAB-MS) was used to rapidly and efficiently identify the peptides in each synthetic mixture which significantly assisted the purification process by HPLC. The method has been successfully applied to the synthesis of magainin 2 and angiotensinogen peptides. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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