| Autor: |
Al Mamun AA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030-3411, USA., Lombardo MJ, Shee C, Lisewski AM, Gonzalez C, Lin D, Nehring RB, Saint-Ruf C, Gibson JL, Frisch RL, Lichtarge O, Hastings PJ, Rosenberg SM |
| Jazyk: |
angličtina |
| Zdroj: |
Science (New York, N.Y.) [Science] 2012 Dec 07; Vol. 338 (6112), pp. 1344-8. |
| DOI: |
10.1126/science.1226683 |
| Abstrakt: |
Mechanisms of DNA repair and mutagenesis are defined on the basis of relatively few proteins acting on DNA, yet the identities and functions of all proteins required are unknown. Here, we identify the network that underlies mutagenic repair of DNA breaks in stressed Escherichia coli and define functions for much of it. Using a comprehensive screen, we identified a network of ≥93 genes that function in mutation. Most operate upstream of activation of three required stress responses (RpoS, RpoE, and SOS, key network hubs), apparently sensing stress. The results reveal how a network integrates mutagenic repair into the biology of the cell, show specific pathways of environmental sensing, demonstrate the centrality of stress responses, and imply that these responses are attractive as potential drug targets for blocking the evolution of pathogens. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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