| Autor: |
McNemar CW; Department of Chemistry, Pennsylvania State University, University Park 16802., Horrocks WD Jr |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 1990 Jan; Vol. 184 (1), pp. 35-8. |
| DOI: |
10.1016/0003-2697(90)90007-v |
| Abstrakt: |
A direct spectroscopic method for the determination of the submicromolar dissociation constant of Eu3+. ATP using laser-induced Eu3+ ion luminescence spectroscopy is described. The dissociation constant of Mg2+.ATP is then determined by the competition of Mg2+ with Eu3+ for the binding of ATP. The experiments were performed in 2H2O to mitigate the significant quenching of the Eu3+ luminescence that occurs in 1H2O. Values for the effective dissociation constants of the 1:1 ATP metal ion complexes of 1.2 +/- 0.3 X 10(-7) and 2.7 +/- 0.7 X 10(-4) M are obtained for Eu3+ and Mg2+, respectively, at p2H 5.8. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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