Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae.

Autor: Ramos SL; Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, CP 83, CEP 13400-970, Piracicaba, São Paulo, Brazil., de Macêdo JL, Lopes MT, Batista JS, Formiga KM, da Silva PP, Saulo-Machado AC, Veasey EA
Jazyk: angličtina
Zdroj: American journal of botany [Am J Bot] 2012 Dec; Vol. 99 (12), pp. e508-10. Date of Electronic Publication: 2012 Dec 04.
DOI: 10.3732/ajb.1100607
Abstrakt: Premise of the Study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. •
Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. •
Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã.
Databáze: MEDLINE
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