Salt-induced pattern formation in evaporating droplets of lysozyme solutions.

Autor: Gorr HM; Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA, USA. hlm32@pitt.edu, Zueger JM, McAdams DR, Barnard JA
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2013 Mar 01; Vol. 103, pp. 59-66. Date of Electronic Publication: 2012 Oct 09.
DOI: 10.1016/j.colsurfb.2012.09.043
Abstrakt: Solute self-organization during evaporation of colloidal sessile droplets has attracted the attention of researchers over the past few decades due to a variety of technological applications. Recently, pattern formation during evaporation of various biofluids has been studied due to potential applications in screening and diagnosis. The complex morphological patterns in the deposit are unique to various disorders and are influenced by various physical mechanisms occurring during evaporation. These complex patterns can be better understood by studying evaporation of model solutions of biological relevance. Here, we examine the general features of pattern formation during sessile droplet evaporation of aqueous lysozyme solutions with varying concentrations of NaCl. Lysozyme is a globular protein found in biological fluids such as tears and saliva. The morphological evolution of the droplet is studied by time-lapse video during evaporation via reflection optical microscopy. The final deposits exhibit an amorphous peripheral ring and interior regions containing crystallites and dendritic forms, dependent on NaCl concentration. Scanning electron microscopy (SEM) images demonstrate the multi-scale hierarchical nature of these structures.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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