Immunodetection and quantification of airborne (1-3)-β-D-glucan-carrying particles with the halogen immunoassay.

Autor: Rivera-Mariani FE; Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States. frivera2@jhmi.edu, Mihalic JN, Rule AM, Breysse PN
Jazyk: angličtina
Zdroj: Journal of immunological methods [J Immunol Methods] 2013 Feb 28; Vol. 388 (1-2), pp. 86-9. Date of Electronic Publication: 2012 Nov 29.
DOI: 10.1016/j.jim.2012.11.009
Abstrakt: Fungal cell wall components, such as (1-3)-β-D-glucan, are known to be capable of activating the innate immune system and pose a respiratory health risk in different environments. Mass-based non-viable techniques commonly used for assessment of fungal exposures could be β-D-glucan-specific, but are limited to analysis of liquid extracts. The variable solubility of different β-D-glucans may underestimate β-D-glucan exposure and long sampling times required for mass-based methods make assessing short-term exposures difficult. In this study, we evaluated the utility of the halogen immunoassay (HIA), an immunoblotting technique previously used for allergens, to immunodetect and quantify β-D-glucan-carrying particles (BGCPs). The HIA was able to detect BGCPs without background staining when β-D-glucan standards and air samples collected at a poultry house during short sampling periods were evaluated. The image analysis protocol previously developed by our group for mouse allergen allowed simultaneous immunodetection and quantification of β-D-glucan-containing particles. Our results suggest that the HIA holds promise for quantifying β-D-glucan exposures. To our knowledge, this is the first time in which the HIA was used for non-allergenic compounds of microbial or fungal origins.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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