Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy.

Autor: Rinaldi L; Department of Animal Pathology and Health, University of Naples Federico II, Naples, Italy. lrinaldi@unina.it, Capasso M, Mihalca AD, Cirillo R, Cringoli G, Cacciò S
Jazyk: angličtina
Zdroj: Parasite (Paris, France) [Parasite] 2012 Nov; Vol. 19 (4), pp. 437-40.
DOI: 10.1051/parasite/2012194437
Abstrakt: In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA) for the detection of Cryptosporidium oocysts and Giardia cysts. IFA revealed the presence of Cryptosporidium oocysts in nine of the 150 samples examined (6.0%), precisely in 6/125 snakes (4.8%) and in 3/25 lizards (12.0%); all fecal samples tested negative for the presence of Giardia cysts. Molecular characterization based on nested PCR amplification and sequencing of the SSU-rRNA gene, revealed the presence of Cryptosporidium serpentis in three samples from snakes (Boa constrictor constrictor, Elapheguttata guttata guttata and Python molurus).
Databáze: MEDLINE