| Autor: |
Balaban NP, Rudakova NL, Sabirova AR, Valeeva LR, Sharipova MR |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2012 Jul-Aug; Vol. 38 (4), pp. 439-48. |
| DOI: |
10.1134/s1068162012040036 |
| Abstrakt: |
Heterologous gene expression of extracellular minor metalloendopeptidase of Bacillus pumilus 3-19 in protease-deficient B. subtilis strain has been studied. The fraction of enzyme in total pool of B. pumilus 3-19 secreted proteases composes less than 8%. The enzyme was isolated from culture liquid of recombinant strain, its primary structure was determined, physicochemical properties were investigated. It was concluded that secreted metallo endopeptidase of B. pumilus 3-19 represents the first prokaryotic homolog of eukaryotic adamalysin/reprolysin protein family. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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