Efficacy of mesoporous silica nanoparticles in delivering BMP-2 plasmid DNA for in vitro osteogenic stimulation of mesenchymal stem cells.

Autor: Kim TH; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea., Kim M, Eltohamy M, Yun YR, Jang JH, Kim HW
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2013 Jun; Vol. 101 (6), pp. 1651-60. Date of Electronic Publication: 2012 Nov 27.
DOI: 10.1002/jbm.a.34466
Abstrakt: We report the ability of aminated mesoporous silica nanoparticles (MSN-NH2) with large mesopore space and positive-charged surface to deliver genes within rat mesenchymal stem cells (MSCs). The amine functionalized inorganic nanoparticles were complexed with bone morphogenetic protein-2 (BMP2) plasmid DNA (pDNA) to study their transfection efficiency in MSCs. Intracellular uptake of the complex BMP2 pDNA/MSN-NH2 occurred significantly, with a transfection efficiency of approximately 68%. Furthermore, over 66% of the transfected cells produced BMP2 protein. The osteogenic differentiation of the transfected MSCs was demonstrated by the expression of bone-related genes and proteins including bone sialoprotein, osteopontin, and osteocalcin. The MSN-NH2 delivery vehicle for BMP2 pDNA developed in this study may be a potential gene delivery system for bone tissue regeneration.
(Copyright © 2012 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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