Re-assessment of the cut-off levels of Carbohydrate Deficient Transferrin (CDT) for automated immunoassay and multi-capillary electrophoresis for application in a forensic context.

Autor: Bortolotti F; Department of Public Health and Community Medicine, Unit of Forensic Medicine, University of Verona, Verona, Italy. federica.bortolotti@univr.it, Trevisan MT, Micciolo R, Canal L, Vandoros A, Palmbach TM, Tagliaro F
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2013 Feb 01; Vol. 416, pp. 1-4. Date of Electronic Publication: 2012 Nov 23.
DOI: 10.1016/j.cca.2012.11.015
Abstrakt: Background: The determination of Carbohydrate Deficient Transferrin (CDT) in a forensic context should be based on the use of a screening technique followed, for the "positive samples", by a confirmatory technique. The aim of this study was to compare the two most used automated screening methods for CDT analysis, immuno-nephelometric assay (INA) and multi-capillary electrophoresis (mCE), with a validated HPLC procedure, used as confirmation test, in order to re-evaluate the cut-off concentrations of the screening methods.
Methods: 195 serum samples underwent CDT analysis by using the N Latex CDT direct immuno-nephelometric assay, the multicapillary system Capillarys™ and an anion exchange HPLC method with UV-visible detection at 460nm developed and validated at our laboratories. Statistical analyses were performed by using Bland-Altman plots and ROC curves.
Results and Discussion: The 95% limits of agreement were ±0.94% when comparing INA and HPLC and ±0.60% when comparing mCE and HPLC. The ROC analysis of both INA and mCE, using HPLC as the reference method, showed that no false negative results were found when the cut-off was fixed to 1.2% for mCE and to 2.3% for INA.
Conclusions: The study showed a good agreement among CDT determinations carried out either with mCE or INA or HPLC. However, the usual cut-offs of both mCE (1.3%) and INA (2.5%) should be lowered to minimize false negatives at the screening analysis.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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