| Autor: |
Surenciski MR; Instituto de Botánica del Nordeste (IBONE), CONICET--UNNE, Facultad de Ciencias Agrarias (UNNE), C.C. 209, (3400) Corrientes, Argentina. surenciski@yahoo.com, Flachsland EA, Terada G, Mroginski LA, Rey HY |
| Jazyk: |
angličtina |
| Zdroj: |
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al [Biocell] 2012 Apr; Vol. 36 (1), pp. 31-6. |
| Abstrakt: |
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30 degrees C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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