| Autor: |
Gautier SM; Laboratoire de Génie Enzymatique-Atelier de Biotechnologie, CNRS-Université Claude Bernard Lyon, Villeurbanne, France., Blum LJ, Coulet PR |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of bioluminescence and chemiluminescence [J Biolumin Chemilumin] 1990 Jan-Mar; Vol. 5 (1), pp. 57-63. |
| DOI: |
10.1002/bio.1170050112 |
| Abstrakt: |
We have investigated highly selective and ultrasensitive biosensors based on luminescent enzyme systems linked to optical transducers. A fibre-optic sensor with immobilized enzymes was designed; the solid-phase bioreagent was maintained in close contact contact with the tip of a glass fibre bundle connected to the photomultiplier tube of a luminometer. A bacterial luminescence fibre-optic sensor was used for the microdetermination of NADH. Various NAD(P)-dependent enzymes, sorbitol dehydrogenase, alcohol dehydrogenase and malate dehydrogenase, were co-immobilized on preactivated polyamide membranes with the bacterial system and used for the microdetermination of sorbitol, ethanol and oxaloacetate at the nanomolar level with a good precision. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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