Chromosomal Aberrations in ETV6/RUNX1-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization.
| Autor: | Zakaria Z; Hematology Unit, Cancer Research Center, Institute for Medical Research, Kuala Lumpur, 50588, Malaysia. zubaidah@imr.gov.my., Ahid MF, Ismail A, Keoh TS, Nor NM, Kamaluddin NR, Esa E, Yuen LK, Rahman EJ, Osman R |
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| Jazyk: | angličtina |
| Zdroj: | Molecular cytogenetics [Mol Cytogenet] 2012 Nov 15; Vol. 5 (1), pp. 41. Date of Electronic Publication: 2012 Nov 15. |
| DOI: | 10.1186/1755-8166-5-41 |
| Abstrakt: | Background: Acute lymphoblastic leukemia (ALL) is a heterogeneous form of hematological cancer consisting of various subtypes. We are interested to study the genetic aberration in precursor B-cell ALL with specific t(12;21) translocation in childhood ALL patients. A high resolution 244K array-based Comparative Genomic Hybridization (array-CGH) was used to study eleven ETV6/RUNX1-positive childhood acute lymphoblastic leukemia (ALL) patients. Result: 155 chromosomal aberrations (119 losses, 36 gains) were reported in the array findings, corresponding to 76.8% deletions and 23.2% amplifications. The ETV6 gene deletion occurred in 4 of the patients, corresponding to 45% of the sample. The most common alterations above 1 Mb were deletion 6q (13%), 12p (12%) and 9p (8%), and duplication 4q (6%) and Xq (4%). Other genes important in ALL were also identified in this study including RUNX1, CDKN2A, FHIT, and PAX5. The array-CGH technique was able to detect microdeletion as small as 400 bp. Conclusion: The results demonstrate the usefulness of high resolution array-CGH as a complementary tool in the investigation of ALL. |
| Databáze: | MEDLINE |
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