| Autor: |
Silva Ados S; Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brasil. alexsantos@ioc.fiocruz.br, Vasconcelos GA, Kappel LA, Pinto MA, Paula VS |
| Jazyk: |
angličtina |
| Zdroj: |
Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2012 Nov; Vol. 107 (7), pp. 960-3. |
| DOI: |
10.1590/s0074-02762012000700022 |
| Abstrakt: |
The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay to measure total anti-HAV antibody levels using anti-HAV IgY as the capture and conjugated immunoglobulins. For this purpose, anti-HAV IgY was conjugated to horseradish peroxidase (HRP) and the optimal dilution of HRP-conjugated antibodies was evaluated to establish the competitive immuneenzymatic assay. The results obtained from our "in-house" assay were plotted on a receiver operator curve, which showed a sensitivity of 95% and a specificity of 98.8%, demonstrating that a competitive anti-HAV IgY immunoenzymatic assay developed "in house" could be used as an alternative to commercial assays that utilise IgG. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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