| Autor: |
Sukhanova AL; Department of Neuroscience, NBICS Center, National Research Center Kurchatov Institute, Laboratory of Neurobiology of Memory, PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia. alsukhanova@gmail.com, Mineyeva OA, Kiselev II, Burtsev MS, Anokhin KV |
| Jazyk: |
angličtina |
| Zdroj: |
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2012 Sep; Vol. 153 (5), pp. 594-7. |
| DOI: |
10.1007/s10517-012-1774-9 |
| Abstrakt: |
We compared the effects of electrical stimulation of primary dissociated neuronal cultures cultured on microelectrode arrays in terms on the expression of c-fos transcriptional factor that is involved in plastic reorganization in neurons. Dissociated hippocampal neurons cultured on multielectrode arrays were exposed to two stimulation protocols: high-frequency and low-frequency stimulations. Expression of c-fos was evaluated using immunofluorescence. Both high-frequency and low-frequency stimulations significantly increased c-fos expression in comparison with non-stimulated control. These findings indicate that c-fos expression can be induced in neuronal cell culture by different types of electrical stimulations and can be used for studying plasticity processes in microphysiological in vitro systems. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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