Rapid, robust method for measuring low concentrations of albumin in urine.

Autor: Medcalf EA; Department of Clinical Biochemistry, London Hospital Medical College, U.K., Newman DJ, Gorman EG, Price CP
Jazyk: angličtina
Zdroj: Clinical chemistry [Clin Chem] 1990 Mar; Vol. 36 (3), pp. 446-9.
Abstrakt: We describe a rapid particle-enhanced turbidimetric immunoassay for albumin in urine. Intra- and interassay CVs were less than 5% and less than 10%, respectively, the detection limit is 2 mg/L, and the working range extends to 200 mg/L. Mean analytical recovery of albumin added to centrifuged urines was 100% (SD 10.6%), and, when results were compared with those by the Pharmacia RIA, the correlation coefficient was 0.99. The working reagents are stable for at least six months; thus this assay is suited for both batch and urgent analysis.
Databáze: MEDLINE