| Autor: |
Ashtiani ZO; Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. ousati@sina.tums.ac.ir, Heidari M, Hasheminasab SM, Ayati M, Rakhshani N |
| Jazyk: |
angličtina |
| Zdroj: |
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2012; Vol. 13 (8), pp. 3863-8. |
| DOI: |
10.7314/apjcp.2012.13.8.3863 |
| Abstrakt: |
In this study mitochondrial D-Loop variations in Iranian prostate cancer and benign prostatic hyperplasia (BPH) patients were investigated. Tumour samples and corresponding non-cancerous prostate tissue from 40 prostate cancer patients and 40 age-matched BPH patients were collected. The entire mtD-loop region (16024-576) was amplified using the PCR method and products were gel-purified and subjected to direct nucleotide sequencing. A total of 129 variations were found, the most frequent being 263A-G and 310T-C among both BPH and prostate cancer patients. Variation of 309 C-T was significantly more frequent in prostate cancer patients (P value<0.05). Four novel variations were observed on comparison with the MITOMAP database. Novel variations were np16154delT, np366G-A, np389G-A and 56insT. There was no correspondence between the different variations and the age of subjects. Considering that D-loop variations were frequent in both BPH and prostate cancer patients in our study, the fact that both groups had high average age can be a possible contributing factor. D-loop polymorphisms and microsatellite instability can influence cell physiology and result in a benign or malignant phenotype. Significantly higher frequency of 309 C-T variation in cancer patients is a notable finding and must be a focus of attention in future studies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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