| Autor: |
Fernandez DI; School of Chemistry, Bio21 Institute, University of Melbourne, Melbourne, VIC 3010, Australia., Le Brun AP, Whitwell TC, Sani MA, James M, Separovic F |
| Jazyk: |
angličtina |
| Zdroj: |
Physical chemistry chemical physics : PCCP [Phys Chem Chem Phys] 2012 Dec 05; Vol. 14 (45), pp. 15739-51. Date of Electronic Publication: 2012 Oct 23. |
| DOI: |
10.1039/c2cp43099a |
| Abstrakt: |
The membrane interactions of the antimicrobial peptide aurein 1.2 were studied using a range of biophysical techniques to determine the location and the mechanism of action in DMPC (dimyristoylphosphatidylcholine) and DMPC/DMPG (dimyristoylphosphatidylglycerol) model membranes that mimic characteristics of eukaryotic and prokaryotic membranes, respectively. Neutron reflectometry and solid-state NMR revealed subtle changes in membrane structure caused by the peptide. Quartz crystal microbalance with dissipation, vesicle dye leakage and atomic force microscopy measurements were used to investigate the global mode of peptide interaction. Aurein 1.2 displayed an enhanced interaction with the anionic DMPC/DMPG membrane while exhibiting primarily a surface interaction with both types of model membranes, which led to bilayer disruption and membrane lysis. The antimicrobial peptide interaction is consistent with the carpet mechanism for aurein 1.2 with discrete structural changes depending on the type of phospholipid membrane. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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