| Autor: |
Levina AS; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia., Repkova MN, Ismagilov ZR, Shikina NV, Malygin EG, Mazurkova NA, Zinov'ev VV, Evdokimov AA, Baiborodin SI, Zarytova VF |
| Jazyk: |
angličtina |
| Zdroj: |
Scientific reports [Sci Rep] 2012; Vol. 2, pp. 756. Date of Electronic Publication: 2012 Oct 22. |
| DOI: |
10.1038/srep00756 |
| Abstrakt: |
Nanoparticles are used to solve the current drug delivery problem. We present a high-performance method for efficient and selective action on nucleic acid target in cells using unique TiO(2)·PL-DNA nanocomposites (polylysine-containing DNA fragments noncovalently immobilized onto TiO(2) nanoparticles capable of transferring DNA). These nanocomposites were used for inhibition of human influenza A (H3N2) virus replication in infected MDCK cells. They showed a low toxicity (TC(50) ≈ 1800 μg/ml) and a high antiviral activity (>99.9% inhibition of the virus replication). The specificity factor (antisense effect) appeared to depend on the delivery system of DNA fragments. This factor for nanocomposites is ten-times higher than for DNA in the presence of lipofectamine. IC(50) for nanocomposites was estimated to be 1.5 μg/ml (30 nM for DNA), so its selectivity index was calculated as ~1200. Thus, the proposed nanocomposites are prospective for therapeutic application. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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