| Autor: |
Vaghchhipawala ZE; Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA., Vasudevan B, Lee S, Morsy MR, Mysore KS |
| Jazyk: |
angličtina |
| Zdroj: |
The Plant cell [Plant Cell] 2012 Oct; Vol. 24 (10), pp. 4110-23. Date of Electronic Publication: 2012 Oct 12. |
| DOI: |
10.1105/tpc.112.100495 |
| Abstrakt: |
Agrobacterium tumefaciens is a soilborne pathogen that causes crown gall disease in many dicotyledonous plants by transfer of a portion of its tumor-inducing plasmid (T-DNA) into the plant genome. Several plant factors that play a role in Agrobacterium attachment to plant cells and transport of T-DNA to the nucleus have been identified, but the T-DNA integration step during transformation is poorly understood and has been proposed to occur via nonhomologous end-joining (NHEJ)-mediated double-strand DNA break (DSB) repair. Here, we report a negative role of X-ray cross complementation group4 (XRCC4), one of the key proteins required for NHEJ, in Agrobacterium T-DNA integration. Downregulation of XRCC4 in Arabidopsis and Nicotiana benthamiana increased stable transformation due to increased T-DNA integration. Overexpression of XRCC4 in Arabidopsis decreased stable transformation due to decreased T-DNA integration. Interestingly, XRCC4 directly interacted with Agrobacterium protein VirE2 in a yeast two-hybrid system and in planta. VirE2-expressing Arabidopsis plants were more susceptible to the DNA damaging chemical bleomycin and showed increased stable transformation. We hypothesize that VirE2 titrates or excludes active XRCC4 protein available for DSB repair, thus delaying the closure of DSBs in the chromosome, providing greater opportunity for T-DNA to integrate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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