| Autor: |
Sakhno LV; Research Institute of Clinical Immunology, Russian Academy of Medical Sciences (RAMS), Siberian Branch (SB), Novosibirsk, Russia. lsahno53@mail.ru, Tikhonova MA, Tyrinova TV, Leplina OY, Shevela EY, Nikonov SD, Zhdanov OA, Ostanin AA, Chernykh ER |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical & developmental immunology [Clin Dev Immunol] 2012; Vol. 2012, pp. 628635. Date of Electronic Publication: 2012 Sep 29. |
| DOI: |
10.1155/2012/628635 |
| Abstrakt: |
The PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure was studied in 76 patients with pulmonary tuberculosis (TB) with normal and low-proliferative response to antigens of M. tuberculosis (purified protein derivative (PPD)). It was revealed that dendritic cells (DCs), generated in vitro from patient blood monocytes with GM-CSF + IFN-α, were characterized by increased B7-H1 expression, upproduction of IL-10, and reducing of allostimulatory activity in mixed lymphocyte culture (MLC). Moreover, DCs of patients with TB were able to enhance T cell apoptosis and to block T-cell division in MLC. It was shown that neutralizing anti-PD1 antibodies significantly decreased the proapoptogenic/tolerogenic effect of DCs. Correlation analysis revealed a direct relationship between IL-10 production and level of B7-H1 expression in the general group of investigated patients. It was demonstrated that generation of healthy donor DCs in the presence of IL-10 led to an increase in the number of DCs-expressed B7-H1 molecule, DC proapoptogenic activity, and a decrease in their allostimulatory activity. Obviously, the revealed phenomenon of the PD-1/B7-H1-mediated pro-apoptogenic activity of DCs is clinically significant since the cytotoxic/tolerogenic potential of DCs is more pronounced in patients with PPD anergy. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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