| Autor: |
Pereira-Júnior OS; Centro de Ciências Agrárias-CCA/UFES, Departamento de Zootecnia, Alto Universitário, s/n Centro, 29500-000, Alegre, Espírito Santo, Brazil., Pereira RV, Silva CS, Castro-Borges W, Sá RG, Cabral FJ, Silva SH, Soares CS, Morais ER, Moreira EB, Magalhães LG, de Paula FM, Rodrigues V |
| Jazyk: |
angličtina |
| Zdroj: |
Parasitology research [Parasitol Res] 2013 Jan; Vol. 112 (1), pp. 235-42. Date of Electronic Publication: 2012 Sep 28. |
| DOI: |
10.1007/s00436-012-3130-4 |
| Abstrakt: |
The ubiquitin-proteasome system is responsible for degradation of the majority of intracellular proteins in eukaryotic cells. The 26S proteasome proteolytic complex is composed of a 20S core particle responsible for protein degradation and the 19S lid which plays a role in the recognition of polyubiquitinated substrates. The 19S regulatory particle (Rps) is composed of ATPase (Rpt) and non-ATPase (Rpn) subunits. In this study, we analyzed the expression profile of 19S Rpt subunits in the larvae and adult stage of the Schistosoma mansoni life cycle. Conventional reverse transcriptase polymerase chain reaction (RT-PCR) revealed that the majority of the 19S Rpt subunits amplified at the expected molecular masses for various investigated stages. In addition, SmRpt1, SmRpt2, and SmRpt6 transcript levels were increased in 3 h-cultured schistosomula and reasonably maintained until 5 h in culture, as revealed by qRT-PCR. Phylogenetic analysis of 19S Rpt subunits showed high structural conservation in comparison to other Rpt orthologues. The mRNA expression profile of 19S Rpt subunits did not correlate with 26S proteasome proteolytic activity as judged by a (14)C-casein-degrading assay, in the early cultured schistosomula. Taken together, these results revealed a differential expression profile for 19S Rpt subunits whose transcript levels could not be directly associated to 26S proteasome activity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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