| Autor: |
Swenberg JA; University of North Carolina, Chapel Hill, NC, USA., Ham AJ, McDorman KS, Morinello EJ, Nakamura J, Schoonhoven R |
| Jazyk: |
angličtina |
| Zdroj: |
Current protocols in toxicology [Curr Protoc Toxicol] 2002 Aug; Vol. Chapter 3, pp. Unit3.8. |
| DOI: |
10.1002/0471140856.tx0308s12 |
| Abstrakt: |
The major event involved in the formation of mutations and the initiation and progression of cancer is the induction of DNA damage by reactive intermediates arising from exposure to endogenous and exogenous chemicals. Many electrophilic metabolites of chemicals covalently bind to the bases of DNA causing specific DNA adducts. This unit includes protocols for preparing samples of intact DNA and adduct analysis to quantify the number of adducts that can potentially cause mutagenic or carcinogenic damage. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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