Immunohistochemical profile of HIF-1α, VEGF-A, VEGFR2 and MMP9 proteins in tegumentary leishmaniasis.

Autor:	Fraga CA; Universidade Estadual de Montes Claros, MG, Brazil., Oliveira MV, Alves LR, Viana AG, Sousa AA, Carvalho SF, De Paula AM, Botelho AC, Guimarães AL
Jazyk:	angličtina
Zdroj:	Anais brasileiros de dermatologia [An Bras Dermatol] 2012 Sep-Oct; Vol. 87 (5), pp. 709-13.
DOI:	10.1590/s0365-05962012000500006
Abstrakt:	Background: Leishmaniasis is one of the most important infectious diseases worldwide. Our study can provide more knowledge about angiogenic and hypoxic events in leishmaniasis. We attempted to verify whether the HIF-1 α protein expression may be associated to VEGF-A, VEGFR2 and MMP9 in leishmanial lesions. Objectives: Besides understanding the pathway, we performed the correlation of VEGF-A, VEGFR2 and MMP9 proteins. Methods: In this study, we gathered 54 paraffin blocks taken from skin lesions in patients from northern Minas Gerais, Brazil, with confirmed diagnosis of tegumentary leishmaniasis. Immunohistochemistry was used to evaluate the expression of the proteins. The expression of HIF-1α was categorized into two groups according to the median: HIF-1 α lower and HIF-1 α higher. Results: We observed increase of VEGFR2 and MMP9 protein expressions in HIF-1 α higher group of epithelial cells. Spearman analyses in epithelial cells showed correlation between VEGF-A and MMP9, VEGFR2 and MMP9 protein expression. Conclusions: HIF-1 α higher group showed increase of VEGFR2 and MMP9 proteins. In epithelial cells, VEGF-A was correlated to MMP9 protein. Furthermore, considering leukocyte cells, VEGFR2 was negatively correlated to MMP9 protein levels. This pathway possibly prepares the cells for a higher activity in a hypoxic or an angiogenic microenvironment. Other in vitro and in vivo studies may clarify the activation mechanism and the response from the proteins HIF-1 α, VEGFR2 and MMP-9 in tegumentary leishmaniasis.
Databáze:	MEDLINE
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