| Autor: |
David S; Instituto Nacional de Saúde Dr. Ricardo Jorge, INSA, IP, Lisbon, Portugal. david.suzana@gmail.com, Sutre AF, Sanca A, Mané A, Henriques V, Portugal C, Sancho L, Cardoso A, Paixão E, Duarte EL, Leite CQ, Salem JI, Antunes A |
| Jazyk: |
angličtina |
| Zdroj: |
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease [Int J Tuberc Lung Dis] 2012 Nov; Vol. 16 (11), pp. 1535-7. |
| DOI: |
10.5588/ijtld.11.0658 |
| Abstrakt: |
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
