A rapid quantitative method of carisoprodol and meprobamate by liquid chromatography-tandem mass spectrometry.

Autor: Essler S; Southwestern University, 1001 E. University Avenue, Georgetown, TX 78626, USA. essler@medicine.tamhsc.edu, Bruns K, Frontz M, McCutcheon JR
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2012 Nov 01; Vol. 908, pp. 155-60. Date of Electronic Publication: 2012 Sep 07.
DOI: 10.1016/j.jchromb.2012.09.001
Abstrakt: The identification and quantitation of carisoprodol (Soma) and its chief metabolite meprobamate, which is also a clinically prescribed drug, remains a challenge for forensic toxicology laboratories. Carisoprodol and meprobamate are notable for their widespread use as muscle relaxants and their frequent identification in the blood of impaired drivers. Routine screening is possible in both an acidic/neutral pH screen and a traditional basic screen. An improvement in directed testing quantitations was desirable over the current options of an underivatized acidic/neutral extraction or a basic screen, neither of which used ideal internal standards. A new method was developed that utilized a simple protein precipitation, deuterated internal standards and a short 2-min isocratic liquid chromatography separation, followed by multiple reaction monitoring with tandem mass spectrometry. The linear quantitative range for carisoprodol was determined to be 1-35mg/L and for meprobamate was 0.5-50mg/L. The method was validated for specificity and selectivity, matrix effects, and accuracy and precision.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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