Expression of DNA methyltransferase genes in four-cell bovine embryos cultured in the presence of oviductal fluid.

Autor: Barrera D; Instituto Superior de Investigaciones Biológicas (INSIBIO), Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina., García EV, Sinowatz F, Palma GA, Jiménez-Díaz MA, Miceli DC
Jazyk: angličtina
Zdroj: Anatomia, histologia, embryologia [Anat Histol Embryol] 2013 Aug; Vol. 42 (4), pp. 312-5. Date of Electronic Publication: 2012 Sep 30.
DOI: 10.1111/ahe.12010
Abstrakt: The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase (DNMT) genes was examined in early bovine embryos (4-cell stage). Real-time quantitative PCR was performed to determine the relative expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression.
(© 2012 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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