Evaluation of Genetic Pattern of Gentamicin-resistant Enterococci Isolated from Clinical Samples.
| Autor: | Balaei Gajan E; Dentist, Department of Community Dentistry, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran., Akbarzadeh P, Harasi B, Moosavi Z |
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| Jazyk: | angličtina |
| Zdroj: | Journal of dental research, dental clinics, dental prospects [J Dent Res Dent Clin Dent Prospects] 2011 Winter; Vol. 5 (1), pp. 23-6. Date of Electronic Publication: 2011 Mar 18. |
| DOI: | 10.5681/joddd.2011.005 |
| Abstrakt: | Background and Aims: Enterococci are among resistant microorganisms to antibiotics and are responsible for some of acute gingival infections. This study aimed at molecular evaluation of gentamicin-resistant enterococcus species and determining the resistance genes. Materials and Methods: Clinical samples were cultured on BHI medium and enterococci were isolated by specific methods and antibiograms were prepared. DNA was derived from gentamicin-resistant species by alkaline lysis method and replicated by PCR method. Four gentamicin-resistant genes were evaluated by electrophoresis method on agarose gel. Results: From 105 Enterococcus faecalis isolates, 36.2% and from 38 Enterococcus faecium isolates, 78.2% were resistant to gentamicin. 60% of these species bore aac (6')- le - aph(2")- la gene. ph(2")- Ib,Ic,Id genes were not seen in any of the evaluated species. Conclusion: In gentamicin-resistant species of E. faecalis and E. faecium, the aac (6')- le - aph(2")- la gene was identified as the main cause of resistance. |
| Databáze: | MEDLINE |
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