| Autor: |
Nikitina LS, Chernigovskaia EV, Dorofeeva NA, Kirillova OD, Glazova MV |
| Jazyk: |
ruština |
| Zdroj: |
Zhurnal evoliutsionnoi biokhimii i fiziologii [Zh Evol Biokhim Fiziol] 2012 Jul-Aug; Vol. 48 (4), pp. 380-5. |
| Abstrakt: |
The antiapoptotic protein Bcl-2 has various functions besides its role in protecting cells from apoptosis. Previous studies have demonstrated that Bcl-2 recruits ERK1/2 and/or CREB to initiate different transcription program in the regulation of various neuronal activities as well as axonal growth. Recently we reported that Bcl-2 can participate in the regulation of synthesis and secretion of vasopressin of rat hypothalamic magnocellular nuclei. In thise study we have investigated the inhibition of Bcl-2 on vasopressin expression in magnocellular neurons of hypothalamic supraoptic nuclei. The experiments were done on short-term incubated rat hypothalamic slices containing supraoptic nuclei. Our data demonstrated that in vitro inhibition of Bcl-2 by HA14-1 prevented CREB translocation into the cell nuclei and significantly decreased vasopressin mRNA level and enhanced contents of vasopressin protein in magnocellular neurons in supraoptic nucleus. Our results indicate that CREB-dependent vasopressin gene transcription in the hypothalamic magnocellular neurons can be regulated by Bcl-2. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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