| Autor: |
Degese MF; Administración Nacional de Laboratorios e Institutos de Salud 'Dr. Carlos G. Malbrán' Instituto Nacional de Enfermedades Infecciosas, Departamento de Parasitología. Av. Vélez Sarsfield 563 (1281) Ciudad Autónoma de Buenos Aires, Argentina. mfdegese@anlis.gov.ar, Cabrera MG, Krivokapich SJ, Irazu LE, Rodríguez MA, Guarnera EA |
| Jazyk: |
Spanish; Castilian |
| Zdroj: |
Revista Argentina de microbiologia [Rev Argent Microbiol] 2012 Apr-Jun; Vol. 44 (2), pp. 97-100. |
| Abstrakt: |
Mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all Latin American countries. It has been described along the Argentine Yungas region. The microscopic diagnosis can yield false-negative test results at low microfilaremia levels. The aim of this study was to optimize the molecular diagnostic technique and compare it with the Knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of individuals from an endemic area. The PCR technique followed by the sequencing of the amplified product yielded 100 % sensitivity compared to the Knott's test, which is considered a reference method. Seven more cases of this parasitosis could only be identified with the molecular technique. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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