| Autor: |
Immer U; R-Biopharm AG, An der neuen Bergstrasse 17, 64297 Darmstadt, Germany. u.immer@r-biopharm.de, Haas-Lauterbach S |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of AOAC International [J AOAC Int] 2012 Jul-Aug; Vol. 95 (4), pp. 1118-24. |
| DOI: |
10.5740/jaoacint.cs2012_01 |
| Abstrakt: |
The Working Group on Prolamin Analysis and Toxicity (WGPAT) organized a collaborative study to confirm whether the two R5 antibody-based ELISA test kits are able to detect gliadin in the lower mg/kg (ppm) level. Twenty laboratories investigated 12 blind-coded samples, spiked and naturally contaminated, to show the possibility of determining traces of gliadin in heat-treated or nonheat-treated foods by ELISA. It was shown that very small amounts of gliadin (below 100 ppm) could be detected by ELISA with a reproducibility RSD(R) (37%) and a repeatability RSD, (27%) common for ELISA under these conditions. The recovery of gliadin from the spiked samples was between 84 and 109%, based on the results of all laboratories, including those with poor performance. No false positives were found by the method (P < or =0.05), but one negative sample was contaminated during the bakery process. It is recommended that the method be accepted by AOAC as Official First Action. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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