Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion.
| Autor: | Girardi SB; Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP), São Paulo, SP, Brazil., Barreto AM, Barreto CC, Proietti AB, Carvalho SM, Loureiro P, Sabino EC |
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| Jazyk: | angličtina |
| Zdroj: | The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases [Braz J Infect Dis] 2012 Sep-Oct; Vol. 16 (5), pp. 452-6. Date of Electronic Publication: 2012 Sep 07. |
| DOI: | 10.1016/j.bjid.2012.08.013 |
| Abstrakt: | The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espírito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (1:50 and 1:100 for the Rapid Test - HIV-1/2 Bio-Manguinhos, and 1:500 and 1:600 for the Rapid Check HIV 1&2). Negative samples were considered recent infection, whereas those showing any color intensity were associated with long-term infection. The best dilutions were 1:100 for HIV-1/2 Bio-Manguinhos test (Kappa = 0.840; overall agreement = 0.93), and 1:500 for the Rapid Check HIV 1&2 (Kappa = 0.867; overall agreement = 0.94). The results suggest that both rapid tests can be used to detect recent seroconversion. (Copyright © 2012 Elsevier Editora Ltda. All rights reserved.) |
| Databáze: | MEDLINE |
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