Effects of purified Saccharomyces cerevisiae (1→3)-β-glucan on venous ulcer healing.

Autor:	Medeiros SDV; Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil., Cordeiro SL; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), 3000, Salgado Filho Ave., Natal, RN 59078-970, Brazil., Cavalcanti JEC; Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil., Melchuna KM; Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil., Lima AMDS; Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil., Filho IA; University Hospital Onofre Lopes, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil., Medeiros AC; University Hospital Onofre Lopes, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil., Rocha KBF; Laboratory of Pathology, Department of Pathology, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil., Oliveira EM; Laboratory of Pathology, Department of Pathology, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil., Faria EDB; University Hospital Onofre Lopes, Department of Integrated Medicine, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil., Sassaki GL; Department of Biochemistry and Molecular Biology, Federal University of Paraná (UFPR), Cento Politécnico S/N, Jardim das Américas, Curitiba, PR 81531-990, Brazil., Rocha HAO; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), 3000, Salgado Filho Ave., Natal, RN 59078-970, Brazil., Sales VSF; Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
Jazyk:	angličtina
Zdroj:	International journal of molecular sciences [Int J Mol Sci] 2012; Vol. 13 (7), pp. 8142-8158. Date of Electronic Publication: 2012 Jul 02.
DOI:	10.3390/ijms13078142
Abstrakt:	Water-insoluble glucan was isolated from the baker's yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healing.
Databáze:	MEDLINE
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