| Autor: |
Certo MT; Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, USA., Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault M, Lambert AR, Baxter SK, Jacoby K, Ryu BY, Kiem HP, Gouble A, Paques F, Rawlings DJ, Scharenberg AM |
| Jazyk: |
angličtina |
| Zdroj: |
Nature methods [Nat Methods] 2012 Oct; Vol. 9 (10), pp. 973-5. Date of Electronic Publication: 2012 Sep 02. |
| DOI: |
10.1038/nmeth.2177 |
| Abstrakt: |
Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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