The immunomodulatory effects of abortion-prone mice decidual and serum soluble factors on macrophages and splenocytes.

Autor: Bibak B; Department of Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran. bibak44@nkums.ac.ir, Gharib FG, Daneshmandi S, Abbaspour AR, Firizi MN, Ahmadabad HN
Jazyk: angličtina
Zdroj: European journal of obstetrics, gynecology, and reproductive biology [Eur J Obstet Gynecol Reprod Biol] 2012 Dec; Vol. 165 (2), pp. 331-6. Date of Electronic Publication: 2012 Aug 31.
DOI: 10.1016/j.ejogrb.2012.08.006
Abstrakt: Objectives: To determine the immune status of the abortion mouse model at the feto-maternal interface and at the systemic level simultaneously.
Study Design: Mid-pregnancy serum and decidual cell supernatants (DS) were obtained from abortion and non-abortion mouse models. The effect of serum and DS on PHA or LPS-induced lymphocyte proliferation was investigated by MTT reduction assay. Treated macrophages and LPS-stimulated macrophages were evaluated for viability and also for nitric oxide (NO) production by Griess reagent.
Results: Our results showed that DS from the abortion mouse model significantly decreased LPS-stimulated splenocyte proliferation, and increased proliferation in PHA-stimulated splenocytes, compared with that in the non-abortion mouse model. Proliferation assays for mid-pregnancy serum were the same on LPS- and PHA-stimulated splenocytes. NO production was decreased by non-abortion DS, similar to that observed for serum treatment in LPS-stimulated macrophages in abortion mice.
Conclusions: These findings suggest that in the abortion mouse model, soluble factors within the decidua are more effective than serum soluble factors in altering immune responses that may be involved in the complex process of fetal rejection.
(Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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