A chemical approach to mapping nucleosomes at base pair resolution in yeast.
| Autor: | Brogaard KR; Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA. Kristin.Brogaard@systemsbiology.org, Xi L, Wang JP, Widom J |
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| Jazyk: | angličtina |
| Zdroj: | Methods in enzymology [Methods Enzymol] 2012; Vol. 513, pp. 315-34. |
| DOI: | 10.1016/B978-0-12-391938-0.00014-8 |
| Abstrakt: | Most eukaryotic DNA exists in DNA-protein complexes known as nucleosomes. The exact locations of nucleosomes along the genome play a critical role in chromosome functions and gene regulation. However, the current methods for nucleosome mapping do not provide the necessary accuracy to identify the precise nucleosome locations. Here we describe a new experimental approach that directly maps nucleosome center locations in vivo genome-wide at single base pair resolution. (Copyright © 2012 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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