A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA).

Autor: Kai J; Siloam Biosciences Inc., 413 Northland Blvd, Cincinnati, USA. Jkai@Siloambio.com, Puntambekar A, Santiago N, Lee SH, Sehy DW, Moore V, Han J, Ahn CH
Jazyk: angličtina
Zdroj: Lab on a chip [Lab Chip] 2012 Nov 07; Vol. 12 (21), pp. 4257-62.
DOI: 10.1039/c2lc40585g
Abstrakt: In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.
Databáze: MEDLINE