Voltage controlled nano-injection system for single-cell surgery.

Autor: Adam Seger R; Department of Biomolecular Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA., Actis P, Penfold C, Maalouf M, Vilozny B, Pourmand N
Jazyk: angličtina
Zdroj: Nanoscale [Nanoscale] 2012 Sep 28; Vol. 4 (19), pp. 5843-6. Date of Electronic Publication: 2012 Aug 16.
DOI: 10.1039/c2nr31700a
Abstrakt: Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses to deliver molecules into individual cells. As a proof of concept, we injected adherent mammalian cells with fluorescent dyes.
Databáze: MEDLINE