Leptospira borgpetersenii from free-living white-eared opossum (Didelphis albiventris): first isolation in Brazil.

Autor: Jorge S; Centro de Controle de Zoonoses, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Hartleben CP, Seixas FK, Coimbra MA, Stark CB, Larrondo AG, Amaral MG, Albano AP, Minello LF, Dellagostin OA, Brod CS
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2012 Nov; Vol. 124 (2), pp. 147-51. Date of Electronic Publication: 2012 Aug 08.
DOI: 10.1016/j.actatropica.2012.07.009
Abstrakt: Leptospirosis is a zoonotic disease that occurs all over the world, caused by bacteria of the genus Leptospira. Marsupial and didelphidae families are considered susceptible to infection caused by a wide range of Leptospira serovars for which they serve as reservoirs. Thirty-three free-living white-eared opossums (Didelphis albiventris) were captured in Southern Brazil and bodily fluids were collected. From the urine samples it was possible to obtain an isolate identified as Leptospira borgpetersenii by rpoB gene sequencing and belonging to serovar Castellonis by Multilocus Variable-Number Tandem-Repeat Analysis. This is the first report of the isolation of Leptospira spp. from the white-eared opossum in Brazil. In addition, the new strain was also virulent in the hamster model of lethal leptospirosis. The microscopic agglutination test (MAT) was used for detecting the presence of antibodies against Leptospira spp. in white-eared opossum, human, cattle and canine sera using a panel of 59 Leptospira strains that included the new isolate. The inclusion of the new strain in the MAT battery increased the MAT sensitivity for canine sera. These findings suggest that the white-eared opossum is an important reservoir of pathogenic Leptospira spp.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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