Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of colonies using the met30 strain.
| Autor: | Tarutina MG; Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny pr., 1, 117545 Moscow, Russia., Dutova TA, Yezhova IE, Nishiuchi H, Sineoky SP |
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| Jazyk: | angličtina |
| Zdroj: | Journal of bioscience and bioengineering [J Biosci Bioeng] 2012 Dec; Vol. 114 (6), pp. 619-21. Date of Electronic Publication: 2012 Aug 11. |
| DOI: | 10.1016/j.jbiosc.2012.07.011 |
| Abstrakt: | Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content. (Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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