[Diagnosis of Down's syndrome using short tandem repeat loci D21S11, D21S1440 and Penta D].

Autor:	Shi YF; Department of Medical Genetics, Tianjin Medical University General Hospital, Tianjin, People's Republic of China., Li XZ, Li Y, Zhang XL, Zhang Y, Yue TF
Jazyk:	čínština
Zdroj:	Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics [Zhonghua Yi Xue Yi Chuan Xue Za Zhi] 2012 Aug; Vol. 29 (4), pp. 443-6.
DOI:	10.3760/cma.j.issn.1003-9406.2012.04.014
Abstrakt:	Objective: To investigate the feasibility of genetic diagnosis of Down's syndrome (DS) using short tandem repeat (STR), and to develop a rapid and accurate method for diagnosing DS. Methods: Quantitative fluorescence polymerase chain reaction (QF-PCR) was used to amplify STR loci D21S11, D21S1440 and Penta D of 719 samples. Three hundred and eighty-nine samples were peripheral blood, 282 were amniotic fluid, 48 were chorionic villous samples. The products were analyzed using eleterophoresis to detect DS. Results: Among 652 samples with a normal karyotype, 635 showed 2 bands with a 1:1 ratio or a single band. The remaining 17 samples showed 3 bands, and were regarded as false positive results. For 67 DS samples, 53 showed 3 bands/peaks with a 1:1:1 ratio and 14 showed 2 bands/peaks with a 2:1 ratio. The sensitivity and specificity of STR loci D21S11, D21S1440 and Penta D were 76.12% and 98.62%, 71.64% and 98.93%, 89.55% and 99.85%, respectively. The overall sensitivity and specificity of 3 STR loci were 100% (67/67) and 97.39% (635/652), respectively. Conclusion: Compared with conventional method, author's method is simpler, more stable and rapid, and can be used for large-scale prenatal screening of DS.
Databáze:	MEDLINE
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