| Autor: |
Lee SH; Animal and Natural Resources Institute, Agricultural Research Service-United States Department of Agriculture, Beltsville, MD 20705, USA., Lillehoj HS, Jang SI, Lee KW, Kim DK, Lillehoj EP, Yancey RJ, Dominowski PJ |
| Jazyk: |
angličtina |
| Zdroj: |
Avian diseases [Avian Dis] 2012 Jun; Vol. 56 (2), pp. 402-5. |
| DOI: |
10.1637/9906-082411-ResNote.1 |
| Abstrakt: |
The effects against avian coccidiosis of two novel adjuvants, Quil A/cholesterol/dimethyl dioctadecyl ammonium bromide/Carbopol (QCDC) and QCDC/Bay R1005 (R)/cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides (CpG ODN [T]) (QCDCRT) emulsified with profilin, a conserved Eimeria recombinant protein, were determined in broiler chickens. Chickens were subcutaneously immunized with isotonic saline (control group), profilin (P), profilin emulsified with QCDC (P-Q), or profilin with QCDCRT (P-QR) at 2 and 9 days post-hatch and orally challenged with 1.0 x 10(4) sporulated oocysts of Eimeria acervulina (EA) at 7 days postimmunization. All profilin-immunized groups showed increased body weight gain when compared to the control group, and the P-QR group had significantly higher body weight gain than did those of the P and P-Q groups following EA challenge infection. All groups immunized with profilin showed significantly decreased intestinal lesions compared with the control group, with the P-QR group showing the lowest intestinal lesions among the profilin-treated groups. Finally, the P-QR group showed greater CD4+/CD8+ and TCR1+/TCR2+ splenocytes and higher antiprofilin serum antibody titers compared with the P and P-Q (or both) groups following EA challenge infection. These results further suggest that vaccination of chickens with profilin, in combination with the QCDCRT adjuvant, may provide a novel control strategy against EA infection in commercial flocks. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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