Anonymous and EST-based microsatellite DNA markers that transfer broadly across the fig tree genus (Ficus, Moraceae).

Autor: Heer K; Institute of Experimental Ecology, University of Ulm, Germany. katrin.heer@uni-ulm.de, Machado CA, Himler AG, Herre EA, Kalko EK, Dick CW
Jazyk: angličtina
Zdroj: American journal of botany [Am J Bot] 2012 Aug; Vol. 99 (8), pp. e330-3. Date of Electronic Publication: 2012 Jul 30.
DOI: 10.3732/ajb.1200032
Abstrakt: Premise of the Study: We developed a set of microsatellite markers for broad utility across the species-rich pantropical tree genus Ficus (fig trees). The markers were developed to study population structure, hybridization, and gene flow in neotropical species.
Methods and Results: We developed seven novel primer sets from expressed sequence tag (EST) libraries of F. citrifolia and F. popenoei (subgen. Urostigma sect. Americana) and optimized five previously developed anonymous loci for cross-species amplification. The markers were successfully tested on four species from the basal subgenus Pharmacosycea sect. Pharmacosycea (F. insipida, F. maxima, F. tonduzii, and F. yoponensis) and seven species of the derived subgenus Urostigma (F. citrifolia, F. colubrinae, F. costaricana, F. nymphaeifolia, F. obtusifolia, F. pertusa, and F. popenoei). The 12 markers amplified consistently and displayed polymorphism in all the species.
Conclusions: This set of microsatellite markers is transferable across the phylogenetic breadth of Ficus, and should therefore be useful for studies of population structure and gene flow in approximately 750 fig species worldwide.
Databáze: MEDLINE