Standardized and automated solid-phase extraction procedures for high-throughput proteomics of body fluids.

Autor: Bladergroen MR; Leiden University Medical Center (LUMC), Department of Parasitology, Biomolecular Mass Spectrometry Unit, Albinusdreef 2, 2300 RC, Leiden, The Netherlands., Derks RJ, Nicolardi S, de Visser B, van Berloo S, van der Burgt YE, Deelder AM
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2012 Dec 21; Vol. 77, pp. 144-53. Date of Electronic Publication: 2012 Jul 26.
DOI: 10.1016/j.jprot.2012.07.023
Abstrakt: In order to balance the speed of analytical sample preparation procedures with mass spectrometry (MS)-based clinical proteomics the application of high-throughput robotic systems for body fluid workup is essential. In this paper we describe the implementation of various solid-phase extraction (SPE) sample preparation protocols on two different platforms, namely: 1) Magnetic bead-based SPE of peptides and proteins from body fluids on a Hamilton liquid handling workstation; 2) Cartridge-based SPE on a SPARK Symbiosis system. All SPE protocols were optimized for MS-based proteomics and compared with respect to obtained peptide- and protein profiles. Throughput numbers that were achieved in a 24 hour time frame for the sample workup procedures were more than 700 samples for the magnetic bead-based method and over 1000 samples for the cartridge-based method.
(Copyright © 2012. Published by Elsevier B.V.)
Databáze: MEDLINE
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