| Autor: |
Shein SA; Department of Medical Nanobiotechnologies, N. I. Pirogov Russian National Research Medical University, Moscow, Russia. atomos@rambler.ru, Gurina OI, Leopol'd AV, Baklaushev VP, Korchagina AA, Grinenko NF, Ivanova NV, Volgina NE, Ryabukhin IA, Chekhonin VP |
| Jazyk: |
angličtina |
| Zdroj: |
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2012 May; Vol. 153 (1), pp. 139-42. |
| DOI: |
10.1007/s10517-012-1662-3 |
| Abstrakt: |
Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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