Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.

Autor: Schreier HJ; Department of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA. Schreier@umbc.edu, Dejtisakdi W, Escalante JO, Brailo M
Jazyk: angličtina
Zdroj: Applied and environmental microbiology [Appl Environ Microbiol] 2012 Oct; Vol. 78 (19), pp. 7120-3. Date of Electronic Publication: 2012 Jul 13.
DOI: 10.1128/AEM.01794-12
Abstrakt: A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.
Databáze: MEDLINE